Results 1 to 12 of 12
Like Tree1Likes
  • 1 Post By shizznizzle

Thread: How to test THC the honest way - Tutorial

  1. #1
    420 Member Medical Marijuana's Avatar
    Join Date
    Mar 2008
    Posts
    0
    Blog Entries
    1

    How to test THC the honest way - Tutorial

    There are many claims out there of strains having very high THC levels. After speaking with a scientist at the Commonwealth Scientific and Industrial Research Organisation (CSIRO), I have been told that the only consistent and honest way to measure THC, CBD, and CBN is to use a test using Liquid Gas Chromatography. Here is some info on that process. I have sourced this from Wikipedia.

    Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen, and the stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside glass or metal tubing, called a column. The instrument used to perform gas chromatographic separations is called a gas chromatograph (also: aerograph, gas separator).

    Gas Chromatography is different from other forms of chromatography (HPLC, TLC, etc.) because the solutions travel through the column in a gas state. The interactions of these gaseous analytes with the walls of the column (coated by different stationary phases) causes different compounds to elute at different times called retention time. The comparsion of these retention times is the analytical power to GC. This makes it very similar to HPLC.
    Contents
    [hide]

    * 1 History
    * 2 GC analysis
    * 3 Physical components
    o 3.1 Autosamplers
    o 3.2 Inlets
    o 3.3 Columns
    o 3.4 Detectors
    * 4 Methods
    o 4.1 Carrier gas selection and flow rates
    o 4.2 Inlet types and flow rates
    o 4.3 Sample size and injection technique
    + 4.3.1 Sample injection
    o 4.4 Column selection
    o 4.5 Column temperature and temperature program
    * 5 Data reduction and analysis
    * 6 Application
    * 7 GCs in popular culture
    * 8 See also
    * 9 References
    * 10 External links

    [edit] History

    Chromatography dates to 1903 in the work of the Russian scientist, Mikhail Semenovich Tswett. German graduate student Fritz Prior developed solid state gas chromatography in 1947. Archer John Porter Martin, who was awarded the Nobel Prize for his work in developing liquid-liquid (1941) and paper (1944) chromatography, laid the foundation for the development of gas chromatography and later produced liquid-gas chromatography (1950).

    [edit] GC analysis

    A gas chromatograph is a chemical analysis instrument for separating chemicals in a complex sample. A gas chromatograph uses a flow-through narrow tube known as the column, through which different chemical constituents of a sample pass in a gas stream (carrier gas, mobile phase) at different rates depending on their various chemical and physical properties and their interaction with a specific column filling, called the stationary phase. As the chemicals exit the end of the column, they are detected and identified electronically. The function of the stationary phase in the column is to separate different components, causing each one to exit the column at a different time (retention time). Other parameters that can be used to alter the order or time of retention are the carrier gas flow rate, and the temperature.

    In a GC analysis, a known volume of gaseous or liquid analyte is injected into the "entrance" (head) of the column, usually using a microsyringe (or, solid phase microextraction fibers, or a gas source switching system). As the carrier gas sweeps the analyte molecules through the column, this motion is inhibited by the adsorption of the analyte molecules either onto the column walls or onto packing materials in the column. The rate at which the molecules progress along the column depends on the strength of adsorption, which in turn depends on the type of molecule and on the stationary phase materials. Since each type of molecule has a different rate of progression, the various components of the analyte mixture are separated as they progress along the column and reach the end of the column at different times (retention time). A detector is used to monitor the outlet stream from the column; thus, the time at which each component reaches the outlet and the amount of that component can be determined. Generally, substances are identified (qualitatively) by the order in which they emerge (elute) from the column and by the retention time of the analyte in the column.

    [edit] Physical components

    [edit] Autosamplers

    The autosampler provides the means to introduce automatically a sample into the inlets. Manual insertion of the sample is possible but is no longer common. Automatic insertion provides better reproducibility and time-optimization.

    Different kinds of autosamplers exist. Autosamplers can be classified in relation to sample capacity (auto-injectors VS autosamplers, where auto-injectors can work a small number of samples), to robotic technologies (XYZ robot VS rotating/SCARA-robot – the most common), or to analysis:

    * Liquid
    * Static head-space by syringe technology
    * Dynamic head-space by transfer-line technology
    * SPME

    Traditionally autosampler manufactures are different from GC manufactures and currently no GC manufacture offers a complete range of autosamplers. Historically, the countries most active in autosampler technology development are the United States, Italy, and Switzerland.

    [edit] Inlets

    The column inlet (or injector) provides the means to introduce a sample into a continuous flow of carrier gas. The inlet is a piece of hardware attached to the column head.

    Common inlet types are:

    * S/SL (Split/Splitless) injector; a sample is introduced into a heated small chamber via a syringe through a septum - the heat facilitates volatilization of the sample and sample matrix. The carrier gas then either sweeps the entirety (splitless mode) or a portion (split mode) of the sample into the column. In split mode, a part of the sample/carrier gas mixture in the injection chamber is exhausted through the split vent.
    * On-column inlet; the sample is here introduced in its entirety without heat.
    * PTV injector; Temperature-programmed sample introduction was first described by Vogt in 1979. Originally Vogt developed the technique as a method for the introduction of large sample volumes (up to 250 µL) in capillary GC. Vogt introduced the sample into the liner at a controlled injection rate. The temperature of the liner was chosen slightly below the boiling point of the solvent. The low-boiling solvent was continuously evaporated and vented through the split line. Based on this technique, Poy developed the Programmed Temperature Vaporising injector; PTV. By introducing the sample at a low initial liner temperature many of the disadvantages of the classic hot injection techniques could be circumvented.
    * Gas source inlet or gas switching valve; gaseous samples in collection bottles are connected to what is most commonly a six-port switching valve. The carrier gas flow is not interrupted while a sample can be expanded into a previously evacuated sample loop. Upon switching, the contents of the sample loop are inserted into the carrier gas stream.
    * P/T (Purge-and-Trap) system; An inert gas is bubbled through an aqueous sample causing insoluble volatile chemicals to be purged from the matrix. The volatiles are 'trapped' on an absorbent column (known as a trap or concentrator) at ambient temperature. The trap is then heated and the volatiles are directed into the carrier gas stream. Samples requiring preconcentration or purification can be introduced via such a system, usually hooked up to the S/SL port.
    * SPME (solid phase microextraction) offers a convenient, low-cost alternative to P/T systems with the versatility of a syringe and simple use of the S/SL port.

    [edit] Columns

    Two types of columns are used in GC:

    * Packed columns are 1.5 - 10 m in length and have an internal diameter of 2 - 4 mm. The tubing is usually made of stainless steel or glass and contains a packing of finely divided, inert, solid support material (eg. diatomaceous earth) that is coated with a liquid or solid stationary phase. The nature of the coating material determines what type of materials will be most strongly adsorbed. Thus numerous columns are available that are designed to separate specific types of compounds.
    * Capillary columns have a very small internal diameter, on the order of a few tenths of millimeters, and lengths between 25-60 meters are common. The inner column walls are coated with the active materials (WCOT columns), some columns are quasi solid filled with many parallel micropores (PLOT columns). Most capillary columns are made of fused-silica with a polyimide outer coating. These columns are flexible, so a very long column can be wound into a small coil.
    * New developments are sought where stationary phase incompatibilities lead to geometric solutions of parallel columns within one column. Among these new developments are:
    o Internally heated microFAST columns, where two columns, an internal heating wire and a temperature sensor are combined within a common column sheath (microFAST);
    o Micropacked columns (1/16" OD) are column-in-column packed columns where the outer column space has a packing different from the inner column space, thus providing the separation behaviour of two columns in one. They can be easily fit to inlets and detectors of a capillary column instrument.

    The temperature-dependence of molecular adsorption and of the rate of progression along the column necessitates a careful control of the column temperature to within a few tenths of a degree for precise work. Reducing the temperature produces the greatest level of separation, but can result in very long elution times. For some cases temperature is ramped either continuously or in steps to provide the desired separation. This is referred to as a temperature program. Electronic pressure control can also be used to modify flow rate during the analysis, aiding in faster run times while keeping acceptable levels of separation.

    The choice of carrier gas (mobile phase) is important, with hydrogen being the most efficient and providing the best separation. However, helium has a larger range of flowrates that are comparable to hydrogen in efficiency, with the added advantage that helium is non-flammable, and works with a greater number of detectors. Therefore, helium is the most common carrier gas used.

    [edit] Detectors

    A number of detectors are used in gas chromatography. The most common are the flame ionization detector (FID) and the thermal conductivity detector (TCD). Both are sensitive to a wide range of components, and both work over a wide range of concentrations. While TCDs are essentially universal and can be used to detect any component other than the carrier gas (as long as their thermal conductivities are different than that of the carrier gas, at detector temperature), FIDs are sensitive primarily to hydrocarbons, and are more sensitive to them than TCD. However, an FID cannot detect water. Both detectors are also quite robust. Since TCD is non-destructive, it can be operated in-series before an FID (destructive), thus providing complementary detection of the same eluents.

    Other detectors are sensitive only to specific types of substances, or work well only in narrower ranges of concentrations. They include:

    * discharge ionization detector (DID)
    * electron capture detector (ECD)
    * flame photometric detector (FPD)
    * Hall electrolytic conductivity detector (ElCD)
    * helium ionization detector (HID)
    * nitrogen phosphorus detector (NPD)
    * mass selective detector (MSD)
    * photo-ionization detector (PID)
    * pulsed discharge ionization detector (PDD)
    * thermal energy analyzer (TEA)

    Some gas chromatographs are connected to a mass spectrometer which acts as the detector. The combination is known as GC-MS. Some GC-MS are connected to an NMR spectrometer which acts as a back up detector. This combination is known as GC-MS-NMR. Some GC-MS-NMR are connected to an infrared spectrophotometer which acts as a back up detector. This combination is known as GC-MS-NMR-IR. It must, however, be stressed this is very rare as most analyses needed can be concluded via purely GC-MS.

    [edit] Methods

    The method is the collection of conditions in which the GC operates for a given analysis. Method development is the process of determining what conditions are adequate and/or ideal for the analysis required.

    Conditions which can be varied to accommodate a required analysis include inlet temperature, detector temperature, column temperature and temperature program, carrier gas and carrier gas flow rates, the column's stationary phase, diameter and length, inlet type and flow rates, sample size and injection technique. Depending on the detector(s) (see below) installed on the GC, there may be a number of detector conditions that can also be varied. Some GCs also include valves which can change the route of sample and carrier flow, and the timing of the turning of these valves can be important to method development.

    [edit] Carrier gas selection and flow rates

    Typical carrier gases include helium, nitrogen, argon, hydrogen and air. Which gas to use is usually determined by the detector being used, for example, a DID requires helium as the carrier gas. When analyzing gas samples, however, the carrier is sometimes selected based on the sample's matrix, for example, when analyzing a mixture in argon, an argon carrier is preferred, because the argon in the sample does not show up on the chromatogram. Safety and availability can also influence carrier selection, for example, hydrogen is flammable, and high-purity helium can be difficult to obtain in some areas of the world. (See: Helium--occurrence and production.)

    The purity of the carrier gas is also frequently determined by the detector, though the level of sensitivity needed can also play a significant role. Typically, purities of 99.995% or higher are used. Trade names for typical purities include "Zero Grade," "Ultra-High Purity (UHP) Grade," "4.5 Grade" and "5.0 Grade."

    The carrier gas flow rate affects the analysis in the same way that temperature does (see above). The higher the flow rate the faster the analysis, but the lower the separation between analytes. Selecting the flow rate is therefore the same compromise between the level of separation and length of analysis as selecting the column temperature.

    With GCs made before the 1990s, carrier flow rate was controlled indirectly by controlling the carrier inlet pressure, or "column head pressure." The actual flow rate was measured at the outlet of the column or the detector with an electronic flow meter, or a bubble flow meter, and could be an involved, time consuming, and frustrating process. The pressure setting was not able to be varied during the run, and thus the flow was essentially constant during the analysis.

    Many modern GCs, however, electronically measure the flow rate, and electronically control the carrier gas pressure to set the flow rate. Consequently, carrier pressures and flow rates can be adjusted during the run, creating pressure/flow programs similar to temperature programs.

    [edit] Inlet types and flow rates

    The choice of inlet type and injection technique depends on if the sample is in liquid, gas, adsorbed, or solid form, and on whether a solvent matrix is present that has to be vaporized. Dissolved samples can be introduced directly onto the column via a COC injector, if the conditions are well known; if a solvent matrix has to be vaporized and partially removed, a S/SL injector is used (most common injection technique); gaseous samples (e.g., air cylinders) are usually injected using a gas switching valve system; adsorbed samples (e.g., on adsorbent tubes) are introduced using either an external (on-line or off-line) desorption apparatus such as a purge-and-trap system, or are desorbed in the S/SL injector (SPME applications).

    [edit] Sample size and injection technique

    [edit] Sample injection

    The real chromatographic analysis starts with the introduction of the sample onto the column. The development of capillary gas chromatography resulted in many practical problems with the injection technique. The technique of on-column injection, often used with packed columns, is usually not possible with capillary columns. The injection system, in the capillary gas chromatograph, should fulfil the following two requirements:

    1. The amount injected should not overload the column.
    2. The width of the injected plug should be small compared to the spreading due to the chromatographic process. Failure to comply with this requirement will reduce the separation capability of the column. As a general rule, the volume injected, Vinj, and the volume of the detecor cell, Vdet, should be about 1/10 of the volume occupied by the portion of sample containing the molecules of interest (analytes) when they exit the column.

    Some general requirements, which a good injection technique should fulfill, are:

    * It should be possible to obtain the column’s optimum separation efficiency.
    * It should allow accurate and reproducible injections of small amounts of representative samples.
    * It should induce no change in sample composition. It should not exhibit discrimination based on differences in boiling point, polarity, concentration or thermal/catalytic stability.
    * It should be applicable for trace analysis as well as for undiluted samples.


    Please help improve this article or section by expanding it.
    Further information might be found on the talk page or at requests for expansion. (February 2007)

    [edit] Column selection
    Please help improve this article or section by expanding it.
    Further information might be found on the talk page or at requests for expansion. (February 2007)

    [edit] Column temperature and temperature program

    The column(s) in a GC are contained in an oven, the temperature of which is precisely controlled electronically. (When discussing the "temperature of the column," an analyst is technically referring to the temperature of the column oven. The distinction, however, is not important and will not subsequently be made in this article.)

    The rate at which a sample passes through the column is directly proportional to the temperature of the column. The higher the column temperature, the faster the sample moves through the column. However, the faster a sample moves through the column, the less it interacts with the stationary phase, and the less the analytes are separated.

    In general, the column temperature is selected to compromise between the length of the analysis and the level of separation.

    A method which holds the column at the same temperature for the entire analysis is called "isothermal." Most methods, however, increase the column temperature during the analysis, the initial temperature, rate of temperature increase (the temperature "ramp") and final temperature is called the "temperature program."

    A temperature program allows analytes that elute early in the analysis to separate adequately, while shortening the time it takes for late-eluting analytes to pass through the column.

    [edit] Data reduction and analysis

    Qualitative analysis:

    Generally chromatographic data is presented as a graph of detector response (y-axis) against retention time (x-axis). This provides a spectrum of peaks for a sample representing the analytes present in a sample eluting from the column at different times. Retention time can be used to identify analytes if the method conditions are constant. Also, the pattern of peaks will be constant for a sample under constant conditions and can identify complex mixtures of analytes. In most modern applications however the GC is connected to a mass spectrometer or similar detector that is capable of identifying the analytes represented by the peaks.

    Quantitive analysis:

    The area under a peak is proportional to the amount of analyte present. By calculating the area of the peak using the mathematical function of integration, the concentration of an analyte in the original sample can be determined. Concentration can be calculated using a calibration curve created by finding the response for a series of concentrations of analyte, or by determining the relative response factor of an analyte. The relative response factor is the expected ratio of an analyte to an internal standard (or external standard) and is calculated by finding the response of a known amount of analyte and a constant amount of internal standard (a chemical added to the sample at a constant concentration, with a distinct retention time to the analyte).

    In most modern GC-MS systems, computer software is used to draw and integrate peaks, and match MS spectra to library spectra.

    [edit] Application

    In general, substances that vaporize below ca. 300 °C (and therefore are stable up to that temperature) can be measured quantitatively. The samples are also required to be salt-free; they should not contain ions. Very minute amounts of a substance can be measured, but it is often required that the sample must be measured in comparison to a sample containing the pure, suspected substance.

    Various temperature programs can be used to make the readings more meaningful; for example to differentiate between substances that behave similarly during the GC process.

    Professionals working with GC analyze the content of a chemical product, for example in assuring the quality of products in the chemical industry; or measuring toxic substances in soil, air or water. GC is very accurate if used properly and can measure picomoles of a substance in a 1 ml liquid sample, or parts-per-billion concentrations in gaseous samples.

    In practical courses at colleges, students sometimes get acquainted to the GC by studying the contents of Lavender oil or measuring the ethylene that is secreted by Nicotiana benthamiana plants after artificially injuring their leaves. These GC analyses hydrocarbons (C2-C40+). In a typical experiment, a packed column is used to separate the light gases, which are then detected with a TCD. The hydrocarbons are separated using a capillary column and detected with an FID. A complication with light gas analyses that include H2 is that He, which is the most common and most sensitive inert carrier (sensitivity is proportional to molecular mass) has an almost identical thermal conductivity to hydrogen (it is the difference in thermal conductivity between two separate filaments in a Wheatstone Bridge type arrangement that shows when a component has been eluted). For this reason, dual TCD instruments are used with a separate channel for hydrogen that uses nitrogen as a carrier are common. Argon is often used when analysing gas phase chemistry reactions such as F-T synthesis so that a single carrier gas can be used rather than 2 separate ones. The sensitivity is less but this is a tradeoff for simplicity in the gas supply.

    [edit] GCs in popular culture

    Movies, books and TV shows tend to misrepresent the capabilities of gas chromatography and the work done with these instruments.

    In the U.S. TV show CSI, for example, GCs are used to rapidly identify unknown samples. "This is gasoline bought at a Chevron station in the past two weeks," the analyst will say fifteen minutes after receiving the sample.

    In fact, a typical GC analysis takes much more time; sometimes a single sample must be run more than an hour according to the chosen program; and even more time is needed to "heat out" the column so it is free from the first sample and can be used for the next. Equally, several runs are needed to confirm the results of a study - a GC analysis of a single sample may simply yield a result per chance (see statistical significance).

    Also, GC does not positively identify most samples; and not all substances in a sample will necessarily be detected. All a GC truly tells you is at which relative time a component eluted from the column and that the detector was sensitive to it. To make results meaningful, analysts need to know which components at which concentrations are to be expected; and even then a small amount of a substance can hide itself behind a substance having both a higher concentration and the same relative elution time. Last but not least it is often needed to check the results of the sample against a GC analysis of a reference sample containing only the suspected substance.

    A GC-MS can remove much of this ambiguity, since the mass spectrometer will identify the component's molecular weight. But this still takes time and skill to do properly.

    Similarly, most GC analyses are not push-button operations. You cannot simply drop a sample vial into an auto-sampler's tray, push a button and have a computer tell you everything you need to know about the sample. According to the substances one expects to find the operating program must be carefully chosen.

    A push-button operation can exist for running similar samples repeatedly, such as in a chemical production environment or for comparing 20 samples from the same experiment to calculate the mean content of the same substance. However, for the kind of investigative work portrayed in books, movies and TV shows this is clearly not the case.

  2. #2
    New Member
    Join Date
    Jul 2007
    Location
    Amsterdamp
    Posts
    20

    Re: How to test THC the honest way - Tutorial

    I'm glad there someting simple and acurate like Cannalytics for growers and breeders, but medical users allsow they can search for cannabis with the right cannabinoids now.
    For years and years whe all depanded on big machines to tell us what canabinoids whe smoke, and yet they cant even find all the cannabinoids like Cannalytics does..

    Alpha Nova, Cannalytics.

    Or here

    CANNALYTICS


    xcuus me for the spelling ,
    high as a kite.........


    greetz & take care

    Dokkie
    It's all about cannabis and it always will be!!
    http://www.regularmagazine.nl
    http://www.regularmagazine.eu
    http://www.facebook.nl/Regular.magazine

    Regular magazine, thé magazine for the global cannabis subcultures, read it, sub us & thump up for Regular magazine

  3. #3
    420 Member ZedRules's Avatar
    Join Date
    Nov 2006
    Location
    Los Angeles, CA
    Posts
    803

    Re: How to test THC the honest way - Tutorial

    I'm going to start referring to Smokin Moose as Professer Moose.
    This sounds pricey for growers, what are the benefits for all this effort and science?
    The only safe and sane medicine in the world is marijuana...it's the law that's the cause of any bad effect.

  4. #4
    420 Member Medical Marijuana's Avatar
    Join Date
    Mar 2008
    Posts
    0
    Blog Entries
    1

    Re: How to test THC the honest way - Tutorial

    Quote Originally Posted by dokkie View Post
    I'm glad there someting simple and acurate like Cannalytics for growers and breeders, but medical users allsow they can search for cannabis with the right cannabinoids now.
    For years and years whe all depanded on big machines to tell us what canabinoids whe smoke, and yet they cant even find all the cannabinoids like Cannalytics does..

    Alpha Nova, Cannalytics.

    Or here

    CANNALYTICS


    xcuus me for the spelling ,
    high as a kite.........


    greetz & take care

    Dokkie
    This method uses Thin Layer Chromatography and is inaccurate. TLC is used in high school labs, and is totally unsuited to give accurate cannabinoid levels.

  5. #5
    New Member
    Join Date
    Jul 2007
    Location
    Amsterdamp
    Posts
    20

    Re: How to test THC the honest way - Tutorial

    accurate cannabinoid levels, for what use? A pissing contest?? Like i'ff got 20% THC, who cares?
    I just need to know what kind of Cannabinoids there are in some strains, so I don't need a gas chromatography to check for cannabinoids. And tell me please why it isn't Accurate to check Cannabis with cannalytics? Cause Dutch proffesers from the university in Wageningen Holland, checked the cannalytics and came up with 0,1% inaccurate(human factor). Well that rock's my boat...
    I analysed like 100ts of cannabis sorts and came up whit exelcly the same THC levels as reguler labs. But whats more interresting, are the new Phenotypes whe discovered. But keep on using that expensive gas chromatography if that does it for U, I don't got that kind of money, and it can't even detect THCV or like 57 other cannabinoids, or any criptics.
    This wasn't spam, just a hint for easy cannabinoids research, for people who sereusly want to know what Cannabinoids are in the Cannabis they smoke, ore people who want to sellect phenotype's by their diverend Cannabinoids combinations.

    take care,


    Dokkie
    It's all about cannabis and it always will be!!
    http://www.regularmagazine.nl
    http://www.regularmagazine.eu
    http://www.facebook.nl/Regular.magazine

    Regular magazine, thé magazine for the global cannabis subcultures, read it, sub us & thump up for Regular magazine

  6. #6
    420 Member Medical Marijuana's Avatar
    Join Date
    Mar 2008
    Posts
    0
    Blog Entries
    1

    Re: How to test THC the honest way - Tutorial

    To each their own. CBD and CBN levels are very difficult to test as they are extremely volatile and exist in very small amounts. CBD and CBN are VERY important to medical users as they play an important part in such things as pain relief and appetite stimulation.
    Thank you for your input.
    Last edited by Medical Marijuana; 06-03-2008 at 02:35 AM.

  7. #7
    New Member
    Join Date
    Jun 2006
    Posts
    3

    Re: How to test THC the honest way - Tutorial

    There a lab I can send a sample?

    I ain't lookin' for fodder for a pissin' contest.
    Just like to know how myStuff stacks up with the Market.

  8. #8
    420 Member Medical Marijuana's Avatar
    Join Date
    Mar 2008
    Posts
    0
    Blog Entries
    1

    Re: How to test THC the honest way - Tutorial

    Check your NORML buddies - they may have a contact at a lab. Or, check out the yellow pages under LGC

  9. #9
    420 Member Trash's Avatar
    Join Date
    Jun 2008
    Posts
    59

    Re: How to test THC the honest way - Tutorial

    If I were trying to actually fine tune a plant, I'd go with the gas chromatography and get my moneys worth in the way of proven accuracy. Most of us aren't in need of it but it is available, and for persons that are investing a lot of time and money in growing and continually improving a plant, it would be worth it.

  10. #10
    New Member
    Join Date
    Jun 2006
    Posts
    3

    Re: How to test THC the honest way - Tutorial

    I hear one of the Ohio colleges will test.
    Not sure which or which process they use.

    BubaWildA(wildabout420) may know

  11. #11
    420 Member shizznizzle's Avatar
    Join Date
    Aug 2012
    Location
    Alpha Centauri
    Posts
    639

    Re: How to test THC the honest way - Tutorial

    Quote Originally Posted by dokkie View Post
    accurate cannabinoid levels, for what use? A pissing contest?? Like i'ff got 20% THC, who cares?
    I just need to know what kind of Cannabinoids there are in some strains, so I don't need a gas chromatography to check for cannabinoids. And tell me please why it isn't Accurate to check Cannabis with cannalytics? Cause Dutch proffesers from the university in Wageningen Holland, checked the cannalytics and came up with 0,1% inaccurate(human factor). Well that rock's my boat...
    I analysed like 100ts of cannabis sorts and came up whit exelcly the same THC levels as reguler labs. But whats more interresting, are the new Phenotypes whe discovered. But keep on using that expensive gas chromatography if that does it for U, I don't got that kind of money, and it can't even detect THCV or like 57 other cannabinoids, or any criptics.
    This wasn't spam, just a hint for easy cannabinoids research, for people who sereusly want to know what Cannabinoids are in the Cannabis they smoke, ore people who want to sellect phenotype's by their diverend Cannabinoids combinations.

    take care,


    Dokkie
    Silly, after he posted all that useful information all you could do was try and start your OWN pissing contest......woefully underequipped though.

    Thank you so much for this post moose I was wondering how this was accurately done.

    Shizzy's Sophmore Grow.......current grow

    Shizzy's Super Soil Grow Take 1 (Like a virgin mix)....first grow


    "Martin Lickert: "What do you do? You join the Mothers and you end up working for Zappa! And he makes you be a creep! You could have played the blues with John Mayall, or far-out exciting jazz with Blood, Sweat & Tears."
    Don Preston: "You really think so?"
    Martin Lickert: "Look, no one'll ever take you seriously after this . . . how can they take you seriously? In this business you either gotta play the blues or sing with a high voice."
    Don Preston: "You're right, I never should have joined the Mothers, oh I . . . I could be a star now, oh . . .""

  12. #12
    New Member
    Join Date
    May 2013
    Posts
    1

    Re: How to test THC the honest way - Tutorial

    where would i purchase one of these testers?