Complete Guide To Using STS To Create Feminized Seeds

Is it possible to to clone a plant already in flower, (monster crop) and then spray to speed up the process some? Will it make any difference in turnaround time? Or possible complicate or hinder the transformation? I have never had issues with monster cropping although have only needed to do it when I decided last minute to keep a strain going. It takes a little while for them to re-veg and start producing normal leaves, but they eventually are fine and normal. I just figured that if the clone was already in bloom the transformation to male may be quicker than waiting for a normal bloom cycle after flipping. Maybe it's won't make a difference, but figured I would ask if anyone had any thoughts.
 
Is it possible to to clone a plant already in flower, (monster crop) and then spray to speed up the process some? Will it make any difference in turnaround time? Or possible complicate or hinder the transformation? I have never had issues with monster cropping although have only needed to do it when I decided last minute to keep a strain going. It takes a little while for them to re-veg and start producing normal leaves, but they eventually are fine and normal. I just figured that if the clone was already in bloom the transformation to male may be quicker than waiting for a normal bloom cycle after flipping. Maybe it's won't make a difference, but figured I would ask if anyone had any thoughts.
Do you mean cutting, rooting, and then spraying? What lighting schedule were you thinking of using during this process?

Keep in mind that STS spraying starts during veg.
 
Do you mean cutting, rooting, and then spraying? What lighting schedule were you thinking of using during this process?

Keep in mind that STS spraying starts during veg.
I was thinking about the Alternate schedule B referred to in the post which says to start to spray the day you flip to flower. Was wondering if you can root a clone that already has female flowers and start spraying with a 12/12 light cycle as soon as it's established if it would speed up the process of reversal any. Again, just a thought. Just seemed logical to me that if the process was based on putting a plant into flower and spraying to reverse the sex, if the plant was already in flower that it might make it change more quickly.
 
I was thinking about the Alternate schedule B referred to in the post which says to start to spray the day you flip to flower. Was wondering if you can root a clone that already has female flowers and start spraying with a 12/12 light cycle as soon as it's established if it would speed up the process of reversal any. Again, just a thought. Just seemed logical to me that if the process was based on putting a plant into flower and spraying to reverse the sex, if the plant was already in flower that it might make it change more quickly.
On the first day of flip the plant is still in veg. I have no idea if you can use STS to reverse a plant already in flower (and staying in flower), which is what you're thinking of doing. Definitely report back here if you decide to give it a go!
 
On the first day of flip the plant is still in veg. I have no idea if you can use STS to reverse a plant already in flower (and staying in flower), which is what you're thinking of doing. Definitely report back here if you decide to give it a go!
That's fine, thanks for the input anyway. I may give it a shot and see what happens. To compare I will also use a couple of normal clones from one of my mother plants and see if there is any difference between the two. If I find anything interesting I will report back.
 
What is the best way to store or can I store unused pollen? In a vacuum sealed bag, in the freezer, refrigerator, etc. How long?
 
That's fine, thanks for the input anyway. I may give it a shot and see what happens. To compare I will also use a couple of normal clones from one of my mother plants and see if there is any difference between the two. If I find anything interesting I will report back.

My from experience, reversing a plant already in flower does not work. There might be male flowers, but they either have no pollen or it is not fertile.
I have recently sprayed an Auto 4 weeks after it was planted, that was a bit too late and I am already getting male flowers without pollen. Hopefully a few will be ok.
 
My from experience, reversing a plant already in flower does not work. There might be male flowers, but they either have no pollen or it is not fertile.
I have recently sprayed an Auto 4 weeks after it was planted, that was a bit too late and I am already getting male flowers without pollen. Hopefully a few will be ok.
Thanks for the feedback. I may give it a try with a couple of small clones with flowers already developed. Nothing lost if it doesn't work. Question though, were all your experiments done with autos? And the pollen you were able to collect just wouldn't produce seeds when applied to a plant already in flower? I'm pretty sure that is what you said, I just want to be sure that I understand correctly.

Thanks again

Ron
 
Just recently started trying it on Autos, all of my previous successful STS runs were done with photoperiod plants (GSC, OG Kush, Frisian Dew). Normal yield for a small plant is between 200 and 300 seeds.
A few times, temperatures were too low for the STS to take effect properly before it was degraded by light. Got like 10 seeds and none germinated.

So the different levels seem to be like this: no male flowers -> no pollen -> infertile pollen -> pollen that produces seeds that don't germinate -> pollen that produces viable seeds but is less potent than from a real male plant -> pollen that is as potent as from a male plant so that you need to take the usual precautions as to not impregnate the entire plant..

Adequate temperature seems to be very important, and spraying before the plant has started flowering (on the same day as changing to 12/12 for a photoperiod plant).
 
I git my clone if Maui Waui to reverse now what is next? She does not seem to be progressing. This is her and her buds,(Or pollen sacs.)
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I git my clone if Maui Waui to reverse now what is next? She does not seem to be progressing. This is her and her buds,(Or pollen sacs.)
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Hi Janet. They should produce some pollen if you're lucky. Can you see any yellow dust under any open pods? That's it if you do. I haven't reversed one yet but have used males. I take a small mirror or anything smooth and easy to clean with a razor blade and hold it under some open pods and tap or shake the pollen out onto the glass. Then I let it dry in a low rh area for a day , then into a small jar with a desiccant.

I pollinate buds at 3 weeks after flip and 5 weeks after pollination the seeds should be ripe. They don't need to be harvested if the plant needs more time but can be. Here's one time I did it..
 
Ok I dont really see anything falling . I have made seed before this is my first time I actually git a plant to reverse. It is my 3rd time trying to reverse a plant. The first 2 were colloidal silver. The first plant just turned silver then died. I think I made it too strong. I tried again it looked like it reversed. There were pollen sacs here and there. I think this one I waited too long. Nothing happened no buds no pollen just nothing. Then after several months she died of old age. This time I bought STS and followed the directions completely. It reversed all right I think I hot carried away this time. Just so I don't have a missed opportunity I put a Maui’s Beast in there with Ms Maui and her fem pollen. I may or may not see the pollen but its there. I have been told by someone that reverses all the time he does not always see the pollen but once the plant gets reversed he always gets seeds with STS. We will see. Just in case a back up plan is in place my Mauis Beast is getting som fem pollen I hope. It just went into flower and has a bunch of budsites.
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These are the other Maui’s Beast I have going they all look super bushy and look more like a clone. Mama was a clone papa was beast mode but it was meant for my white widow. I pollinated her inside but was afraid to wash her off when I brought her outside in the fall. I can grow until mid Dec outside here. It was starting to get colder at night so I was worried about bud rot. They are very nice looking girls. I planted 10. All 10 were female.
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Hi all,

according to this meaningful 2018 study ...

and this source ...

..as well as the study they are based on from the early 80s, the STS should be prepared with a molar ratio of 1:4.
If my calculations are correct, that would mean a weight ratio of 5.835:1 for the two components using the Pentahydrate version.

Many older tutorials seem to have taken the second source with its 20/80 solution mix and just quadrupled the amount of Silver Nitrate to get the same amount of A/B solutions, but this lowers the total dosage by a factor of 2.5 because the additional water also dilutes the other component accordingly! However, the very high amount of 20 mM would then still come out as 8 mM, or 0.8 mM after 1:9 dilution. Good enough, but not perfect, as the first source above suggests.

For the ~3 mM solution from the study with nearly 100% success, that would be 0.1g Silver Nitrate and 0.58g Sodium Thiosulfate mixed with 100 ml of distilled water each (=2.92 mM), NOT diluted any further.

Application is done 3 times: once directly before switching 12/12, once a week later, once another week later.

I have found this to work perfectly as long as temperatures are not too low. I did have problems with plants in a basement at 14°C. No problems at 20°+ and complete conversion.

The amount of pollen starts very small but gets larger later. What helps a lot is drying the sacks or, later, the entire plant, because otherwise moisture from the plant tissue soaks up the little pollen there is. For this, flowering has to be induced a few weeks earlier than for the one that is to be pollinated, but just a week earlier works as well.
Thanks a bunch for sharing this useful info; I've been searching everywhere for the 3 mM formula, you are blessed.

So just to clarify: once you've made the 0.1g Silver Nitrate mixed with 100 ml water and 0.58g Sodium Thiosulfate (pentahydrate, right?) mixed with 100 ml water, you then mix both on a 1/1 ratio? So let's say combine both to make 200 ml and use that directly on the plants without further dilution? Am I reading you correct?

Sorry, two questions in one with the Pentahydrate!
 
Oh and btw about this :

"Application is done 3 times: once directly before switching 12/12, once a week later, once another week later."

If the study you mention is the same as the one I'm thinking about (attached), it recommends one single application 7 days prior to flip (with 3nM solution ratio 1:4 as you explained). You are recommending 3 so I was wondering; have you found one single application prior flip not to be enough? Are you getting better results with 3 applications/3nM?

thank you
 

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Yes, the mixture is made just like you said.
In the study I linked, they applied it 3 times. I have not tested using it just once, but I would expect the conversion to be incomplete with a mix of male and female flowers. Maybe a higher concentration would work with a single application.
 
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