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How To Reverse Sex Using Silver Thiosulfate Solution

tony11

New Member
The following is a safe, inexpensive, and successful method for reversing the sex of female cannabis plants. Individual plant responses may vary based upon strain, but I can verify that this process is fully effective in stimulating profuse staminate flower production.

This process can be used to:
A: create new feminized seeds from solitary prize mothers that you currently have
B: create interesting feminized-seed hybrids from different prize strains that you currently have
C: create feminized seeds for optimum outdoor use
D: accelerate the "interview" phase of cultivation, in searching for interesting new clone-mothers
E: reduce total plant numbers- great for medical users with severe plant number restrictions
F: increase variety, by helping to create stable feminized seedlines to be used as an alternative to clones

At the bottom of this post are some specific details about the chemicals used, their safety, their cost, and where to get them.

It is important to educate yourself about cannabis breeding theory and technique prior to using a method like this one. Here is a link to Robert Clarke's "Marijuana Botany", which is a very good reference.

"Marijuana Botany" by Robert Connell Clarke
(unfortunately missing the appendices)

It is also important to use basic safety precautions when mixing and handling these chemicals, so read the safety data links provided. The risk is similar to mixing and handling chemical fertilizers, and similar handling procedures are sufficient.

Remember: nothing will ever replace good genetics, and some of your bounty should always go back towards the professional cannabis breeders out there... the ones who have worked for many generations to come up with their true-breeding F1 masterpieces. Support professional breeders by buying their seeds. Also, order from Heaven's Stairway. Not that they need a plug from me, but they are very professional and provide very fast service worldwide.

Preparation of STS:
First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

Part A: .5 gram silver nitrate stirred into 500ml distilled water
Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water

The silver nitrate dissolves within 15 seconds. The sodium thiosulfate takes 30-45 seconds to dissolve.

The silver nitrate solution (A) is then mixed into the sodium thiosulfate solution (B) while stirring rapidly. The resulting blend is stock silver thiosulfate solution (STS).

This stock solution is then diluted at a ratio of 1:9 to make a working solution. For example, 100ml of stock STS is added to 900ml of distilled water. This is then sprayed on select female plants.

Both the stock STS and the working solution should be refrigerated after use, as well as the powdered chemicals, to avoid activity loss. Excess working solution can be safely poured down the drain after use (with ample running water) with negligible environmental impact. It's pretty cheap.

Each liter of stock STS will make ten 1-liter batches of working solution of STS. With the minimum amount of base chemicals ordered from Photographer's Formulary (see link below), this means that each 1-liter bottle of working solution STS costs less than 9 cents, and can treat 15-20 mid-sized plants. That's 200 1-liter batches of STS for $18. Note that the distilled water costs far more than the chemicals.

Application:
The STS working solution is sprayed on select female plants until runoff. Do the spraying over newspaper in a separate area from the flower room. You probably won't smell anything, but ventilate anyway. You now have what I call a "F>M plant"; a female plant that will produce male flowers.

After the F>M plant dries move it into 12/12 immediately. This is usually done three to four weeks prior to the date that the target (to be pollinated) plants will be ready to pollinate. Response times may vary slightly depending upon the strain. More specific times can be determined by trial with your own individual strains. In my trials it took 26 days for the first pollen. 30-35 days seems optimum for planning purposes.

So, assuming that a target plant needs 3-4 weeks to produce fully mature seeds, a strain that takes 8 weeks to mature should be moved into flower at about the same time as the female>male plant. A target plant that finishes flowering in 6 weeks needs to be moved into flower later (10 days or so) so that it doesn't finish before the seeds can fully mature.

A seeded individual branch can be left to mature on a plant for a bit longer, while harvesting the other seedless buds if they finish first. Just leave enough leaves on for the plant for it to stay healthy.

Effects:
Within days I noticed a yellowing of the leaves on the F>M plants. This effect persisted for two weeks or so; after this they became green again, except for a few of the larger fans. The plants otherwise seemed healthy. No burning was observed. Growth stopped dead for the first ten days, and then resumed slowly. No stretch was ever seen. After two weeks the F>M plants were obviously forming male flower clusters. Not just a few clusters of balls, but complete male flower tops. One plant still formed some pistillate flowers, but overall it was predominantly male.

It is strange indeed to see an old girlfriend that you know like the back of your hand go through a sex change. I'll admit that things were awkward between us at first.

When the F>M plants look like they may soon open and release pollen, ( 3-1/2 to 4 weeks) move them from the main flower room into another unventilated room or closet with lighting on a 12/12 timer. Don't worry too much about watts per square foot; it will only be temporary.

When the pollen flies, move your target plants into the closet and pollinate.

A more controlled approach is to isolate the F>M plants in a third remote closet (no light is necessary in this one, as they are releasing pollen now and are nearly finished anyway). In this remote other closet the pollen is very carefully collected in a plastic produce bag or newspaper sleeve and then brought back to the lighted closet, where the target plants are now located. If this is done, be careful to not mix pollen types by letting the F>Ms dust each other. Avoid movement, or use yet another closet.

Take special care to not let pollen gather on the outside of this bag- a static charge is sometimes present. Drop small open clusters of blooms inside and then close the bag at the mouth and shake. Important: next, step outside and slowly release the excess air from the bag, collapsing it completely, so that pollen doesn't get released accidently. Point downwind; don't let it get on your hands or clothes.

This collapsed pollinated bag is now very carefully slipped over only one branch and is then tied off tightly at the mouth around the branch stem with a twist tie or tape, sealing the pollen inside. Let the bag inflate slightly with air again before sealing it off, so the branch can breathe. This technique keeps the entire plant from seeding. Agitate the bag a bit after tying it off to distribute the pollen. Don't forget to label the branch so you know which seeds are which. Other branches on this same plant can be hit with different pollen sources.

If no lighted closet is available, the plant can be moved back into the main room, but- be very carefulollen is sneaky. After 4-5 days, the bag is gently removed and the plant completes it's flowering cycle.

Yet another method has worked well for me. I position the target plants in a non-ventilated lighted closet, and then I collect pollen on a piece of mirror or glass. This is then carefully applied to the pistils of one pre-labeled branch by using a very fine watercolor paintbrush. Care is taken to not agitate the branch or the pollen. No sneezing. The plant needs to be in place first; moving it after pollination can shake pollen free and blow this technique.

Regardless of technique, at completion you will have feminized seeds. Let them dry for 2-4 weeks.

About the chemicals:
Silver nitrate is a white crystalline light-sensitive chemical that is commonly used in photography. It is also used in babies' eyes at birth to prevent blindness. It can cause mild skin irritation, and it stains brown. Avoid breathing. I didn't notice any smell or fumes, but ventilation is recommended. Be sure to wash the spray bottle well before you use it elsewhere; better yet: devote a bottle to STS use. A half gram is a surprisingly small amount; it would fit inside a gel capsule.

Here are links to some safety data. A Google search will bring up more information if needed.

Silver Nitrate info:
ICSC:NENG1116 International Chemical Safety Cards (WHO/IPCS/ILO) | CDC/NIOSH
http://www.lions.odu.edu/~redwards/... solution.pdf

For a realistic hazard level comparison, here is a link for the safety and handling data for Ammonium Nitrate, or common fertilizer:

Sodium thiosulfate is also a white crystalline chemical commonly used in photography; it is used in photographic fixers. Same general cautions apply, minus the staining. This formula uses the anhydrous type. Non-hazardous.

Sodium Thiosulfate info:
http://ptcl.chem.ox.ac.uk/MSDS/SO/s...hiosulfate.html
http://www.med-chem.com/MSDS/Sodium_Thiosulf.htm

------------------
Where to get the chemicals:

Photographic chemicals, photo chemistry, photo processing equipment, photo chemicals

silver nitrate: 10 grams: $10
http://www.photoformulary.com/Deskt...yID=27&langID=0

sodium thiosulfate (anhydrous): 100 grams: $3.95
http://www.photoformulary.com/Deskt...yID=28&langID=0

Postage runs around $4. Fast service. Can be shipped to Canada.

Have fun experimenting with this technique. Use it responsibly. There are a few good threads here at CW that go into the pros and cons of transsexual agents and feminized seeds. Read them. And most importantly, use STS with quality F1 strains developed by professional breeders for the most consistent results.

A huge thanks to Fet from Spice Brothers Seeds for his help and advice in using this technique. I simply brought together available information from previous posts and tried my own recipe. I'm thrilled to share the results. Future tests will be done to adjust the formula so the molar ratios of the chemicals are correct, as specified by Gobgoober (thanks, Gob) but the formula posted here is completely effective.
can reverse plant sex be done outdoor or no???? it is for strict indoor thing???/
:thumb:
 

Cannabelle

New Member
Indoor, away from the flowering room or outdoor crop, is safest to avoid accidental pollination of your entire bud crop. Yes you can reverse sex outdoor female branch, but you need to control that one male branch from pollinating your entire bud crop.

If you must grow outdoors, make an indoor clone. It would not need intense lighting. 2 CFLs with 100w output is enough to grow 1 20-30 inch plant for seed only.


Isolating a branch to spray
:green_heart:
Cannabelle's Isolated Seed Stock Area

 
Last edited:

Jacobsenji

Well-Known Member
The following is a safe, inexpensive, and successful method for reversing the sex of female cannabis plants. Individual plant responses may vary based upon strain, but I can verify that this process is fully effective in stimulating profuse staminate flower production.

This process can be used to:
A: create new feminized seeds from solitary prize mothers that you currently have
B: create interesting feminized-seed hybrids from different prize strains that you currently have
C: create feminized seeds for optimum outdoor use
D: accelerate the "interview" phase of cultivation, in searching for interesting new clone-mothers
E: reduce total plant numbers- great for medical users with severe plant number restrictions
F: increase variety, by helping to create stable feminized seedlines to be used as an alternative to clones

At the bottom of this post are some specific details about the chemicals used, their safety, their cost, and where to get them.

It is important to educate yourself about cannabis breeding theory and technique prior to using a method like this one. Here is a link to Robert Clarke's "Marijuana Botany", which is a very good reference.

"Marijuana Botany" by Robert Connell Clarke
(unfortunately missing the appendices)

It is also important to use basic safety precautions when mixing and handling these chemicals, so read the safety data links provided. The risk is similar to mixing and handling chemical fertilizers, and similar handling procedures are sufficient.

Remember: nothing will ever replace good genetics, and some of your bounty should always go back towards the professional cannabis breeders out there... the ones who have worked for many generations to come up with their true-breeding F1 masterpieces. Support professional breeders by buying their seeds. Also, order from Heaven's Stairway. Not that they need a plug from me, but they are very professional and provide very fast service worldwide.

Preparation of STS:
First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

Part A: .5 gram silver nitrate stirred into 500ml distilled water
Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water

The silver nitrate dissolves within 15 seconds. The sodium thiosulfate takes 30-45 seconds to dissolve.

The silver nitrate solution (A) is then mixed into the sodium thiosulfate solution (B) while stirring rapidly. The resulting blend is stock silver thiosulfate solution (STS).

This stock solution is then diluted at a ratio of 1:9 to make a working solution. For example, 100ml of stock STS is added to 900ml of distilled water. This is then sprayed on select female plants.

Both the stock STS and the working solution should be refrigerated after use, as well as the powdered chemicals, to avoid activity loss. Excess working solution can be safely poured down the drain after use (with ample running water) with negligible environmental impact. It's pretty cheap.

Each liter of stock STS will make ten 1-liter batches of working solution of STS. With the minimum amount of base chemicals ordered from Photographer's Formulary (see link below), this means that each 1-liter bottle of working solution STS costs less than 9 cents, and can treat 15-20 mid-sized plants. That's 200 1-liter batches of STS for $18. Note that the distilled water costs far more than the chemicals.

Application:
The STS working solution is sprayed on select female plants until runoff. Do the spraying over newspaper in a separate area from the flower room. You probably won't smell anything, but ventilate anyway. You now have what I call a "F>M plant"; a female plant that will produce male flowers.

After the F>M plant dries move it into 12/12 immediately. This is usually done three to four weeks prior to the date that the target (to be pollinated) plants will be ready to pollinate. Response times may vary slightly depending upon the strain. More specific times can be determined by trial with your own individual strains. In my trials it took 26 days for the first pollen. 30-35 days seems optimum for planning purposes.

So, assuming that a target plant needs 3-4 weeks to produce fully mature seeds, a strain that takes 8 weeks to mature should be moved into flower at about the same time as the female>male plant. A target plant that finishes flowering in 6 weeks needs to be moved into flower later (10 days or so) so that it doesn't finish before the seeds can fully mature.

A seeded individual branch can be left to mature on a plant for a bit longer, while harvesting the other seedless buds if they finish first. Just leave enough leaves on for the plant for it to stay healthy.

Effects:
Within days I noticed a yellowing of the leaves on the F>M plants. This effect persisted for two weeks or so; after this they became green again, except for a few of the larger fans. The plants otherwise seemed healthy. No burning was observed. Growth stopped dead for the first ten days, and then resumed slowly. No stretch was ever seen. After two weeks the F>M plants were obviously forming male flower clusters. Not just a few clusters of balls, but complete male flower tops. One plant still formed some pistillate flowers, but overall it was predominantly male.

It is strange indeed to see an old girlfriend that you know like the back of your hand go through a sex change. I'll admit that things were awkward between us at first.

When the F>M plants look like they may soon open and release pollen, ( 3-1/2 to 4 weeks) move them from the main flower room into another unventilated room or closet with lighting on a 12/12 timer. Don't worry too much about watts per square foot; it will only be temporary.

When the pollen flies, move your target plants into the closet and pollinate.

A more controlled approach is to isolate the F>M plants in a third remote closet (no light is necessary in this one, as they are releasing pollen now and are nearly finished anyway). In this remote other closet the pollen is very carefully collected in a plastic produce bag or newspaper sleeve and then brought back to the lighted closet, where the target plants are now located. If this is done, be careful to not mix pollen types by letting the F>Ms dust each other. Avoid movement, or use yet another closet.

Take special care to not let pollen gather on the outside of this bag- a static charge is sometimes present. Drop small open clusters of blooms inside and then close the bag at the mouth and shake. Important: next, step outside and slowly release the excess air from the bag, collapsing it completely, so that pollen doesn't get released accidently. Point downwind; don't let it get on your hands or clothes.

This collapsed pollinated bag is now very carefully slipped over only one branch and is then tied off tightly at the mouth around the branch stem with a twist tie or tape, sealing the pollen inside. Let the bag inflate slightly with air again before sealing it off, so the branch can breathe. This technique keeps the entire plant from seeding. Agitate the bag a bit after tying it off to distribute the pollen. Don't forget to label the branch so you know which seeds are which. Other branches on this same plant can be hit with different pollen sources.

If no lighted closet is available, the plant can be moved back into the main room, but- be very carefulollen is sneaky. After 4-5 days, the bag is gently removed and the plant completes it's flowering cycle.

Yet another method has worked well for me. I position the target plants in a non-ventilated lighted closet, and then I collect pollen on a piece of mirror or glass. This is then carefully applied to the pistils of one pre-labeled branch by using a very fine watercolor paintbrush. Care is taken to not agitate the branch or the pollen. No sneezing. The plant needs to be in place first; moving it after pollination can shake pollen free and blow this technique.

Regardless of technique, at completion you will have feminized seeds. Let them dry for 2-4 weeks.

About the chemicals:
Silver nitrate is a white crystalline light-sensitive chemical that is commonly used in photography. It is also used in babies' eyes at birth to prevent blindness. It can cause mild skin irritation, and it stains brown. Avoid breathing. I didn't notice any smell or fumes, but ventilation is recommended. Be sure to wash the spray bottle well before you use it elsewhere; better yet: devote a bottle to STS use. A half gram is a surprisingly small amount; it would fit inside a gel capsule.

Here are links to some safety data. A Google search will bring up more information if needed.

Silver Nitrate info:
ICSC:NENG1116 International Chemical Safety Cards (WHO/IPCS/ILO) | CDC/NIOSH
404 Not Found

For a realistic hazard level comparison, here is a link for the safety and handling data for Ammonium Nitrate, or common fertilizer:

Sodium thiosulfate is also a white crystalline chemical commonly used in photography; it is used in photographic fixers. Same general cautions apply, minus the staining. This formula uses the anhydrous type. Non-hazardous.

Sodium Thiosulfate info:
http://ptcl.chem.ox.ac.uk/MSDS/SO/s...hiosulfate.html
http://www.med-chem.com/MSDS/Sodium_Thiosulf.htm

------------------
Where to get the chemicals:

Photographic chemicals, photo chemistry, photo processing equipment, photo chemicals

silver nitrate: 10 grams: $10
Photographers Formulary - Not Found

sodium thiosulfate (anhydrous): 100 grams: $3.95
Photographers Formulary - Not Found

Postage runs around $4. Fast service. Can be shipped to Canada.

Have fun experimenting with this technique. Use it responsibly. There are a few good threads here at CW that go into the pros and cons of transsexual agents and feminized seeds. Read them. And most importantly, use STS with quality F1 strains developed by professional breeders for the most consistent results.

A huge thanks to Fet from Spice Brothers Seeds for his help and advice in using this technique. I simply brought together available information from previous posts and tried my own recipe. I'm thrilled to share the results. Future tests will be done to adjust the formula so the molar ratios of the chemicals are correct, as specified by Gobgoober (thanks, Gob) but the formula posted here is completely effective.
Gonna Borrow this Brutha. Great post and thank you very much
 

joeyjose

New Member
About the use of Silver Thiosulfate Solution to turn female to male.
Sorry the flowing 2 questions can be a little stupid.
1) Should I spray Silver Thiosulfate Solution once a day for target plant EVERYDAY until male sac shows?
2) My plant is a auto-flower strain that flower initiation shower 38 days after germination (10 weeks to harvest, recommended by seed bank), when show I start to do Silver Thiosulfate Solution treatment on it?
 

bestbudz420

New Member
Hey can you help me determine what gender my plants are?? I know I have at least 3 female plants jus not sure of others.

Sent from my 5054N using 420 Magazine Mobile App
 

Nismo12

Member of the Month: May 2017 - Grow Journal of the Month: Dec 2016
About the use of Silver Thiosulfate Solution to turn female to male.
Sorry the flowing 2 questions can be a little stupid.
1) Should I spray Silver Thiosulfate Solution once a day for target plant EVERYDAY until male sac shows?
2) My plant is a auto-flower strain that flower initiation shower 38 days after germination (10 weeks to harvest, recommended by seed bank), when show I start to do Silver Thiosulfate Solution treatment on it?
Spray twice in one day once a week. So for us we spray on Sundays twice. Once at mid day and once after lights out. A total of 6 applications is what you need.

As for when to start, as soon as you see pistils on an auto or right around 4 weeks from germination is when you should start spraying, which ever comes first.

I just did a write up in our perpetual journal on Sunday to help people out if anything was unclear. Super easy to do. Hope it all goes well for ya.
Hey can you help me determine what gender my plants are?? I know I have at least 3 female plants jus not sure of others.

Sent from my 5054N using 420 Magazine Mobile App
Show them off and we will tell ya. :thumb:
 

GrizzWald

Plant of the Month: June 2016 - Nug of the Month: Aug 2017
Reverse sex :19::19::19:
 

TorturedSoul

Member of the Month: May 2009, Oct 2010, Sept 2017
Better and faster than colloidal silver, IMHO. But a little harder to come by now that the digital camera / cell phone thing has all but driven local photography shops out of business.
 

Blazinjones

Well-Known Member
Jus got some sts and this thread might have possibly saved my seed collection lol ive been trying colloidal silver n it only worked on one plant for a 30 dollar bottle n for 24 bux n change im guna be able 2 make gallons upon gallons of this stuff lol so way better deal imo.
 

Blazinjones

Well-Known Member
The following is a safe, inexpensive, and successful method for reversing the sex of female cannabis plants. IndividuNon-hazardous.

A huge thanks to Fet from Spice Brothers Seeds for his help and advice in using this technique. I simply brought together available information from previous posts and tried my own recipe. I'm thrilled to share the results. Future tests will be done to adjust the formula so the molar ratios of the chemicals are correct, as specified by Gobgoober (thanks, Gob) but the formula posted here is completely effective.
Thanx so much for thorwing this up i can now finally start making my own fem seeds. I started this round withtryn my hand agian at autos i used c.s (colloidal silver) last time an got a bunch of pollen of a few autos none on others but then no luck getting seeds. Atleast nt fully developed ones anyway. So now this time with the sts im doing 2 autos and 1 photo. The autos are pineapple express and c4 from fast buds and then the photo is devil's carnival so wish me luck every1.
 

Pennywise

Member of the Year: 2017 - Member of the Month: Mar & Oct 2017, Aug 2018, May 2019 - Plant of the Month: Aug 2017
I think timing is everything, Moonshine from Rare Dank did a YouTube video on picking the right time to pollinate your girls. You may have pollen before the female is ready. May have to collect pollen then freeze it until the right moment.
 

Blazinjones

Well-Known Member
I think timing is everything, Moonshine from Rare Dank did a YouTube video on picking the right time to pollinate your girls. You may have pollen before the female is ready. May have to collect pollen then freeze it until the right moment.
Yea what is the best way to hold on to pollen so it dnt go bad? I no u need to let it sit out for abot to dry then what? Ive always jus tried to get the timming of when one girl is ready to recieve pollen to have one finish makn pollen at the same time.
 

Pennywise

Member of the Year: 2017 - Member of the Month: Mar & Oct 2017, Aug 2018, May 2019 - Plant of the Month: Aug 2017
I keep mine in a glass vial with a few raw rice grains to absorb moisture. Then I store it in the freezer, you can add flour to it to make the pollen stretch farther.
 

Blazinjones

Well-Known Member
I keep mine in a glass vial with a few raw rice grains to absorb moisture. Then I store it in the freezer, you can add flour to it to make the pollen stretch farther.
Id prefer not to do the flower incase it messes it up further lol but i got organic n a vial i cud use so hopefully its all good. How long did u let it sit out b4 u put it in the vial an into freezer?
 

Pennywise

Member of the Year: 2017 - Member of the Month: Mar & Oct 2017, Aug 2018, May 2019 - Plant of the Month: Aug 2017
The male flowers dried for a couple days before I collected it, the raw rice will pull any moisture from the pollen.
 

Blazinjones

Well-Known Member
The male flowers dried for a couple days before I collected it, the raw rice will pull any moisture from the pollen.
Awesome thanx man. I cant wait to start making auto beans. I think member if memory serves right that after u make ur first successful auto bean dont u have to cross it again with another one of the autos to secure the auto trait in? Now does that mean i cud use one of tye beans i made as long as it was one of the autos right?
 

Pennywise

Member of the Year: 2017 - Member of the Month: Mar & Oct 2017, Aug 2018, May 2019 - Plant of the Month: Aug 2017
2 autos will pass along the auto trait in all of the offspring. Cross an auto with a photo plant and all offspring will be photos. The auto trait is recessive and photo is dominate. Breed to F1 siblings from an auto x photo pairing and the result will be 50% photos, 25% autos and 25% fast. Choose 2 autos from that generation and breed them and you'll get 100% autos.
 

Blazinjones

Well-Known Member
2 autos will pass along the auto trait in all of the offspring. Cross an auto with a photo plant and all offspring will be photos. The auto trait is recessive and photo is dominate. Breed to F1 siblings from an auto x photo pairing and the result will be 50% photos, 25% autos and 25% fast. Choose 2 autos from that generation and breed them and you'll get 100% autos.
Awesome man thanx im guna copy this in my breeding journal i got on hand for quick references lol.
 

Pennywise

Member of the Year: 2017 - Member of the Month: Mar & Oct 2017, Aug 2018, May 2019 - Plant of the Month: Aug 2017
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