420 Magazine Background

Post your Polyploids

MyDogSpot

Well-Known Member
Definition:

Polyploid cells and organisms are those containing more than two paired (homologous) sets of chromosomes. Most species whose cells have nuclei (Eukaryotes) are diploid, meaningthey have two sets of chromosomes–one set inherited from each parent.
~ Wikipedia

Because of the limited resources I personally have found, I'm hoping to gather a few more pictures and examples of polyploidy as it relates to cannabis.
I've been scouring the web looking for other examples and while I did find a few, I didn't want to jack anyone photos from other forums, to post here. Plus I believe that we have at least two polyploid plants growing right now in journals on 420magazine forums. So let's make a resource for the next folks that see this mutation.

So please if you would, post your examples of this that you have come across, or share the information you may have, with the rest of us :)
I certainly don't expect too many to post pics as this is a rare mutation.
 

MyDogSpot

Well-Known Member
I'll start off with the pictures I've taken of mine. She's in flower right now as I wasn't aware what this was at first. Had I known probably would not have put her to flower yet.

This is my example of a hexaploid, (6 sets of chromosomes!


The first two pictures are pre-flower.
Check out the flat stem that is an inch wide and an 1/8"-1/4" thick. Bizarre.
And last but not least check out the crazy bud formation. 6-in-1.
 

MyDogSpot

Well-Known Member
There are so many fan leaves. Layers and layers of them, randomly all around the stem and from the main flower mutation. They are very close together as well, which makes sense considering the 6 tops growing together.
There are a variety of leaf mutations as well and many of the fan leaves, have only 3 points.



Only 2 weeks into flower and this stack of nodes is building fast!
 

TaylorMade420

Well-Known Member
Whole plants went nuts, second time its happened in 2 grows from same seed supplier buds grown on last plant weren't smokeable took 6 weeks after switching to 12/12 to show flowers, pretty decent shatter from it tho just a shame about the extra time i had to spend flowering it
 

MyDogSpot

Well-Known Member
Whole plants went nuts, second time its happened in 2 grows from same seed supplier buds grown on last plant weren't smokeable took 6 weeks after switching to 12/12 to show flowers, pretty decent shatter from it tho just a shame about the extra time i had to spend flowering it
Very cool!
Ans did you happen to count how many tops you have on there?
Thanks for posting.
May I ask...
Same seed supplier, same strain?
I'd love to know the what and where ;)
 

coralman

Well-Known Member
I cant count how many nodes on mine theres that many but theyv all interwined at the top and made like a huge ball of growth, the picture dosnt do it justice, then all down the shoot which about an inch wide new nodes are sprouting like random all around the main stem. Iv just flipped to 12/12 and took 3 clones off her. Lets see what she brings....
Pictures are from now back until she was about 2 weeks old.
 

coralman

Well-Known Member
Colchicine treatment is done in one of the following ways:

a. Seed treatment:

The dry or soaked seeds are soaked in aqueous solution of colchicine of different strength in shallow container to facilitate aeration (generally, aqueous solutions of 0.25 to 0.5 per cent concentrations are used). Colchicine treatment is given for a definite period which is different for different seeds.

After the seeds are soaked in colchicine solution for desired period, they are washed thoroughly in water and then they are sown. Treatment of dry seeds gives better result than soaked seeds in some cases.

b. Seedling treatment:

Seedlings may be treated in young stage. During treatment, the shoot tips are dipped in 0.2% colchicine solution and root tips are covered with cotton soaked in water and the treatment may be given from 3 to 24 hours and in some cases the treatment should be repeated on 2nd and 3rd day also.

c. Treatment of growing buds of shoot:

In some cases growing points are treated with 0.1 to 0.5% solution of colchicine which is applied by brush or with a dropper. Sometimes cotton wool soaked in the aqueous solution of colchicine is applied over the growing point of plant.

The treatment is repeated once or twice daily for a few days. Alternatively, 0.2 to 0.5% colchicine solution is mixed with lanoline paste and is smeared on the shoot apex. This treatment may be repeated 2-3 times daily for a week.

d. In woody plants, 1.0% colchicine solution may be applied over the growing bud. For proper penetration of colchicine, the solution is applied 2-3 times a day for a week.

At any given time only a small proportion of cells would be in mitotic phase, hence repeated treatment should be given at brief intervals so as to double the chromosome number in good number of cells. There may be a competition between polyploid cells and unaffected diploid cells and some-times diploid cells may compete over the polyploid cells and diploid shoots may arise from the treated growing apex.

Effect of Colchicine:

When colchicine is applied some irregularities occur in the mitotic division. It has no effect on doubling of chromosomes which proceeds in normal fashion. This chemical is a spindle poison and it simply checks the anaphasic movement of chromosomes to the two poles and consequently mitosis fails to complete.

The doubled chromosomes become bounded by a nuclear membrane, thus a restitution nucleus with double chromosome number is formed.
 

MyDogSpot

Well-Known Member
I wanted to post this pic because it explains in a bat of an eye, exactly what a polyploid is.
A plant would normally have just three chromosomes and would be called a haploid.


This image shows haploid (single), diploid (double), triploid (triple), and tetraploid (quadruple) sets of chromosomes. Triploid and tetraploid chromosomes are examples of polyploidy.

Stolen from Wikipedia
---------------------
 

bignugz420

Well-Known Member
I wanted to post this pic because it explains in a bat of an eye, exactly what a polyploid is.



This image shows haploid (single), diploid (double), triploid (triple), and tetraploid (quadruple) sets of chromosomes. Triploid and tetraploid chromosomes are examples of polyploidy.

Stolen from Wikipedia
---------------------
I think my plant is doing somethings similar to this is stalk is huge and it's new growth looks crazy I'll post a picture soon
 

bignugz420

Well-Known Member
Here is the one I have some weird growth to, she is still veg it's gold rush by canuck seeds, which is Tangie/Kosher kush
never had a stalk so thick in a month old plant
 

MyDogSpot

Well-Known Member
Im having a hard time seeing exactly, a mutation in those pics.
Maybe in was the third pic, but those dang fans leaves are in the way :p
 
Top Bottom