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Abstract
OBJECTIVE
To screen the AFLP primers with good diversity to distinguish various species of Cannabis.
METHODS
The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.
RESULTS
A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.
CONCLUSION
AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.
Source: Unbound MEDLINE : [DNA polymorphism detection of Cannabis using amplified fragment length polymorphism
OBJECTIVE
To screen the AFLP primers with good diversity to distinguish various species of Cannabis.
METHODS
The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.
RESULTS
A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.
CONCLUSION
AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.
Source: Unbound MEDLINE : [DNA polymorphism detection of Cannabis using amplified fragment length polymorphism
