Jacob Bell
New Member
Gamma-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells
Gallily R, Even-Chena T, Katzavian G, Lehmann D, Dagan A, Mechoulam R.
Source
The Lautenberg Center for General and Tumor Immunology, The Hebrew University Medical Faculty, Ein Kerem Campus, Jerusalem 1120, Israel. rgallily@md.huji.ac.il
Abstract
Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 microg/ml CBD and 15 microg/ml CBD-DMH, respectively, after a 24 h treatment. Prior exposure of the cells to gamma-irradiation (800 cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or gamma-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.
Source: Gamma-Irradiation Enhances Apoptosis Induced by Cannabidiol, a Non-Psychotropic
Gallily R, Even-Chena T, Katzavian G, Lehmann D, Dagan A, Mechoulam R.
Source
The Lautenberg Center for General and Tumor Immunology, The Hebrew University Medical Faculty, Ein Kerem Campus, Jerusalem 1120, Israel. rgallily@md.huji.ac.il
Abstract
Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 microg/ml CBD and 15 microg/ml CBD-DMH, respectively, after a 24 h treatment. Prior exposure of the cells to gamma-irradiation (800 cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or gamma-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.
Source: Gamma-Irradiation Enhances Apoptosis Induced by Cannabidiol, a Non-Psychotropic
