Julie Gardener
New Member
Effect of Ethanol Extracts of Three Chinese Medicinal Plants with Laxative Properties on Ion Transport of the Rat Intestinal Epithelia
Jong-Chang TSAI, a Shuli TSAI, b and Weng-Cheng CHANG*, b,c a
Department of Physical Education, National Changhua University of Education; Changhua, 500 Taiwan; b Department of Physiology, China Medical University; and c Department of Sports Medicine, China Medical University; 91 Hsueh-Shih Road, Taichung, 404 Taiwan. Received April 25, 2003; accepted October 10, 2003
The effects of ethanol extracts of three Chinese medicinal plants Dahuang (Rheum palmatum L.), Badou (Croton tiglium L.), and Huomaren (Cannabis sativa L.), on ion transport of the rat intestinal epithelia were studied. Rat intestinal epithelia mounted in an Ussing chamber attached with voltage/current clamp were used for measuring changes of the short-circuit current across the epithelia. The intestinal epithelia were activated with current raised by serosal administration of forskolin 5 M. Ethanol extracts of the three plants all augmented the current additively when each was added after forskolin. In subsequent experiments, ouabain and bumetanide were added prior to ethanol extracts of these medicinal plants to determine their effect on Na1 and Cl2 movement. The results suggest that ethanol extracts of the three medicinal plants may affect the Cl2 movement more directly than Na1 movement in the intestinal epithelial cells. The results provide evidence for the pharmacologic mechanism of the three Chinese medicinal plants on the intestinal tract.
Source: Effect of Ethanol Extracts of Three Chinese Medicinal Plants with Laxative Properties on Ion Transport of the Rat Intestinal Epithelia
Jong-Chang TSAI, a Shuli TSAI, b and Weng-Cheng CHANG*, b,c a
Department of Physical Education, National Changhua University of Education; Changhua, 500 Taiwan; b Department of Physiology, China Medical University; and c Department of Sports Medicine, China Medical University; 91 Hsueh-Shih Road, Taichung, 404 Taiwan. Received April 25, 2003; accepted October 10, 2003
The effects of ethanol extracts of three Chinese medicinal plants Dahuang (Rheum palmatum L.), Badou (Croton tiglium L.), and Huomaren (Cannabis sativa L.), on ion transport of the rat intestinal epithelia were studied. Rat intestinal epithelia mounted in an Ussing chamber attached with voltage/current clamp were used for measuring changes of the short-circuit current across the epithelia. The intestinal epithelia were activated with current raised by serosal administration of forskolin 5 M. Ethanol extracts of the three plants all augmented the current additively when each was added after forskolin. In subsequent experiments, ouabain and bumetanide were added prior to ethanol extracts of these medicinal plants to determine their effect on Na1 and Cl2 movement. The results suggest that ethanol extracts of the three medicinal plants may affect the Cl2 movement more directly than Na1 movement in the intestinal epithelial cells. The results provide evidence for the pharmacologic mechanism of the three Chinese medicinal plants on the intestinal tract.
Source: Effect of Ethanol Extracts of Three Chinese Medicinal Plants with Laxative Properties on Ion Transport of the Rat Intestinal Epithelia
