JohnnieJC3
Well-Known Member
Similar process... you go to AGAR from the spores, I like to transfer once or twice, if it looks clean I'll take a piece for another AGAR plate, and dump the whole plate in one jar. If it's(in the jar) contaminated, you will usually see it within 3 days. You have to sterilize the blocks 2.5 hours and let them cool for 8 hours. You can leave them in the PC if you aren't doing anything else, or I stick them in a clean Alcohol sprayed box if I have more duties with the PC. I warn you, it's a little more involved process, but I KNOCK up two blocks per quart jar of spawn at a time. It's a tight fight in a SAB, I have a little over 100 quart tub for the SAB and it's still takes getting used to. I've done 34 blocks so.... It's just like anything else in life, (repeating a process), I'm fast and efficient now.Ok i had a question about that actually. With a sawdust block in a bag can i do agar with those to inoculate them or do they have to be done with a syringe? Cause im guessing i wont be able 2 do the shiitake in grain jars right? Dont i shoot up a sawdust block or wud i do a grain jar like normal an then add the grains to the sawdust block at the same time i add it to the unicorn bags? Im jus tryn to figure out how do i continue my shiitake mushrooms and also my oysters once i get there prints?
Fold your blocks underneath themselves and have the filter patches facing each other in the PC, if the filters are facing outward(towards outer edge of PC instead of in the middle), you run the risk of the bag getting caught underneath the trivet. Also, if your bag or bags are close to the metal bar inside the PC that connects the locking mechanism(seals the PC), shut it down before it fully pressurizes by flipping the bobber. IT won't effect the block since it's not even close to 15lbs yet, but if that doesn't seal correctly, it will leak and won't pressurize properly. When inoculating blocks, turn the block on it's side, you will know what I mean if and when you do this... Your Welcome in Advance!
Also shake spawn jars in advance so they are just ready, then spray them down with alcohol when you are about to put them in the SAB. I try to slide the jar in between both bag flaps and wrap the bag around the glass (on it's side in the SAB) use my other hand to pour in the Oats, slide jar out while closing up the bag around the glass and twist the top of the bag(make sure you get all sides of the bag in the twist). Grab your zip tie that is on top of the SAB while holding the twist with your other hand. You will get there, just be patient, you have a window of opportunity as long as you sprayed everything down well(70% IS0). All the Best!